Polymorphic Sites Away from the Bw4 Epitope That Affect Interaction of Bw4+ HLA-B with KIR3DL1

Polymorphic sites away from the Bw4 epitope that affect interaction of Bw4+ HLA-B with KIR3DL1.

KIR3DL1 is a polymorphic, inhibitory NK cell receptor specific for the Bw4 epitope carried by subsets of HLA-A and HLA-B allotypes. The Bw4 epitope of HLA-B*5101 and HLA-B*1513 is determined by the NIALR sequence motif at positions 77, 80, 81, 82, and 83 in the alpha(1) helix. Mutation of these positions to the residues present in the alternative and nonfunctional Bw6 motif showed that the func...

Cell Inhibition by HLA-Bw4 Ligand KIR3DL1 Polymorphisms That Affect NK

KIR3DL1 polymorphisms that affect NK cell inhibition by HLA-Bw4 ligand.

The killer cell Ig-like receptor (KIR) gene family encodes MHC class I receptors expressed by NK cells and several T cell subpopulations. Factors contributing to human KIR haplotype diversity are differences in gene number, gene content, and allelic polymorphism. Whereas functional and clinical consequences of the first two factors are established, knowledge of the effects of KIR gene polymorph...

Alloantibody to a Bw4 epitope in a Bw4+B*27: 05 patient.

BACKGROUND Alloantibodies to the Bw4 epitope are known to be heterogeneous, but it is widely assumed that anti-Bw4 alloantibodies arise only in individuals who do not express a Bw4 epitope. METHODS Bw4 expression was confirmed by DNA sequence analysis. Anti-Bw4 reactivity was confirmed by absorption with transfected cells. RESULTS A Bw4 (B*27:05 or B*27:13) patient expressed antibody that b...

Interactions of NK cell receptor KIR3DL1*004 with chaperones and conformation-specific antibody reveal a functional folded state as well as predominant intracellular retention.

Variable interaction between the Bw4 epitope of HLA-B and the polymorphic KIR3DL1/S1 system of inhibitory and activating NK cell receptors diversifies the development, repertoire formation, and response of human NK cells. KIR3DL1*004, a common KIR3DL1 allotype, in combination with Bw4(+) HLA-B, slows progression of HIV infection to AIDS. Analysis in this study of KIR3DL1*004 membrane traffic in...